Fig. 1

Standard curves for Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pneumoniae, Pseudomonos aeruginosa, Haemophilus influenzeae, and Moraxella catarrhalis assays. Mean threshold cycle (CT) value ± SEM of three replicates (three per run) for each reaction is shown in relation to the log of CFU calculated per 20 μl of reaction mixture. Relative correlation coefficient (R²) and linear regression equations are reported in the legend