Table 1 RT-qPCR primers and assay performance summary

Staphylococcus aureus

egc

F:5′-CTTCATATGTGTTAAGTCTTGCAGCTT-3′

R² = 0.995

[12]

R:5′-TTCACTCGCTTTATTCAATTGTTCTG-3′

Slope = −3.91

P: 5′-6-FAM -ATGTTAAATGGCAATCCT-TAMRA -3′

Efficiency = 1.04

Klebsiella pneumoniae

khe

F: 5′-GATGAAACGACCTGATTGCATTC-3′

R² = 0.997

[11]

R: 5′-CCGGGCTGTCGGGATAAG-3′

Slope = −3.60

P:5′-6-FAM-CGCGAACTGGAAGGGCCCG-TAMRA-3′

Efficiency = 1.07

Streptococcus pneumoniae

cps

F: 5′-GCTGTTTTAGCAGATAGTGAGATCGA-3′

R² = 0.995

[13]

R: 5′-TCCCAGTCGGTGCTGTCA-3′

Slope = −3.11

P: 5′-6-FAM-AATGTTACGCAACTGACGAG-TAMRA -3′

Efficiency = 1.12

Pseudomonos aeruginosa

gyrB

F: 5′-GGCGTGGGTGTGGAAGTC-3′

R² = 0.995

[14]

R: 5′-TGGTGGCGATCTTGAACTTCTT-3′

Slope = −3.55

P: 5′-6-FAM-TGCAGTGGAACGACA-TAMRA-3′

Efficiency = 0.96

Haemophilus influenzeae

omp P6

F:5′-CCAGCTGCTAAAGTATTAGTAGAAG-3′

R² = 0.997

[9]

R: 5′-TTCACCGTAAGATACTGTGCC-3′

Slope = −4.22

P: 5′-6-FAM -CAGATGCAGTTGAAGGTTATTTAG-

Efficiency = 0.95

TAMRA-3′

Moraxella catarrhalis

copB

F: 5′-GTGAGTGCCGCTTTACAACC-3′

R² = 0.998

[10]

R: 5′-TGTATCGCCTGCCAAGACAA-3′

Slope = −3.66

P:5′-6-FAM-TGCTTTTGCAGCTGTTAGCCAGCCTAA-

Efficiency = 0.91

TAMRA-3′