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Table 1 RT-qPCR primers and assay performance summary

From: Quantitative analysis of pathogens in the lower respiratory tract of patients with chronic obstructive pulmonary disease

Organism Target gene Primer/Probe sequences Assay linearity Ref.
Staphylococcus aureus egc F:5′-CTTCATATGTGTTAAGTCTTGCAGCTT-3′ R2 = 0.995 [12]
R:5′-TTCACTCGCTTTATTCAATTGTTCTG-3′ Slope = −3.91
P: 5′-6-FAM -ATGTTAAATGGCAATCCT-TAMRA -3′ Efficiency = 1.04
Klebsiella pneumoniae khe F: 5′-GATGAAACGACCTGATTGCATTC-3′ R2 = 0.997 [11]
R: 5′-CCGGGCTGTCGGGATAAG-3′ Slope = −3.60
P:5′-6-FAM-CGCGAACTGGAAGGGCCCG-TAMRA-3′ Efficiency = 1.07
Streptococcus pneumoniae cps F: 5′-GCTGTTTTAGCAGATAGTGAGATCGA-3′ R2 = 0.995 [13]
R: 5′-TCCCAGTCGGTGCTGTCA-3′ Slope = −3.11
P: 5′-6-FAM-AATGTTACGCAACTGACGAG-TAMRA -3′ Efficiency = 1.12
Pseudomonos aeruginosa gyrB F: 5′-GGCGTGGGTGTGGAAGTC-3′ R2 = 0.995 [14]
R: 5′-TGGTGGCGATCTTGAACTTCTT-3′ Slope = −3.55
P: 5′-6-FAM-TGCAGTGGAACGACA-TAMRA-3′ Efficiency = 0.96
Haemophilus influenzeae omp P6 F:5′-CCAGCTGCTAAAGTATTAGTAGAAG-3′ R2 = 0.997 [9]
R: 5′-TTCACCGTAAGATACTGTGCC-3′ Slope = −4.22
P: 5′-6-FAM -CAGATGCAGTTGAAGGTTATTTAG- Efficiency = 0.95
TAMRA-3′  
Moraxella catarrhalis copB F: 5′-GTGAGTGCCGCTTTACAACC-3′ R2 = 0.998 [10]
R: 5′-TGTATCGCCTGCCAAGACAA-3′ Slope = −3.66
P:5′-6-FAM-TGCTTTTGCAGCTGTTAGCCAGCCTAA- Efficiency = 0.91
TAMRA-3′