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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells

Fig. 2

a Observation of serial sections of a whole paraffin block. Its 3D trapezoid size was approximately 0.7×0.5×0.4×0.4 mm. Serial cuts resulted in 250 sections with a thickness of 2 μm. The 1st -50th and 201st -250th sections were considered to be identical (same marginal condition), as well as the 51st -100th and 151st -200th sections (same para-peripheral condition). To compare incidents of infected cells among tissue, every odd numbered slides from 1st-50th (peripheral region, N = 25) and 101st-150 (central region, N = 25) were utilized. b Distribution of infected cells in different layers of the tissue. The x-axis shows the number of sections, the y-axis the numbers of infected cells. Descriptive statistics showed that the peripheral slides were associated with a number of infected cells 47.87 ± 38.9, whereas, central slides had 126.92 ± 59.47 of infected cells with high variances, followed by inferential statistics (non-parametric Mann–Whitney test)

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