Fig. 5
From: Role of CD248 as a potential severity marker in idiopathic pulmonary fibrosis
CD248 expression do not changes significantly in A549 (n = 3) or in primary type II alveolar epithelial cells (ATII, n = 6) upon TGF-β1 – induced EMT. Cells were treated with 10 ng/ml TGF-β1 for 72 h. mRNA expression levels of CD248, SLUG Vimentin (VIM) and GAPDH were tested with reverse transcription quantitative PCR (RT-qPCR). On the graph, reverse dCt values are plotted; values were calculated as described above. CD248 expression levels were very low and did not changed significantly upon growth factor treatments. In contrast, expression levels of EMT markers SLUG and VIM were significantly elevated (*p < 0.05) in the TGF-β1-treated samples, indicating that epithelial cells have undergone EMT