Fig. 4
Lung morphological changes in bi-transgenic mice. a Lungs from left and inferior lobes were collected at PN21. b Lung size from INSM1/rtTA bi-transgenic mice (Bi-TG) in all lobes was compared to that of wild type control littermates, and single transgenic mice. The lung size was measured by weight scale after the lung was inflated with 10 % formalin via trachea. Bi-transgenic mice and the control littermates were treated with doxycycline from E0 to the time when lungs were sampling. Lung sections were stained with H&E on E17.5 (c, d) and PN21 (e, f). The original magnification is 200X. Air space area (mm2) was measured at E17.5 and PN21 with Kodak MI image system. Value are mean ± SE. P < 0.001 by one way Anova and Student’s t-test