Fig. 4

HuR affected the levels and stability of ICAM-1 mRNA without influencing IL-8 mRNA in HPMECs. Following TNF-α stimulation, the ICAM-1 and IL-8 mRNA levels were significantly increased in control cells, while HuR knockdown markedly reduced ICAM-1 mRNA levels but not IL-8 mRNA (a and b). HuR silencing decreased ICAM-1 mRNA stability, and the half-life was 118 min and 52 min in control cells and HuR-silenced cells, respectively (c). The half-life of IL-8 mRNA was 126 min and 114 min in control cells and HuR-silenced cells, respectively (d). Each point represents the mean ± SEM. n =3, #P <0.05 vs. negative control siRNA-transfected cells, *P <0.05 vs. cells at TNF-α 0 h