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Table 2 EGFR mutations detected by SNapShot, qPCR and NGS

From: Comparison of next generation sequencing, SNaPshot assay and real-time polymerase chain reaction for lung adenocarcinoma EGFR mutation assessment

ID Tumor cell content (%) Identified mutations
qPCR SNaPShot NGS
DNA change Designation Total Coverage (%, number of reads)
BM 40 60 T790M T790M c.2369C > T, T790M, 40 % (141),
   L858R L858R c.2573 T > G L858R 23 % (60)
BM 49 25 L858R L858R c.2573 T > G L858R 38 % (321)
BM 65 10 L858R none Not detected by automatic analysis   
BM 71 6 DEL 19 DEL 19 c.2236_2250delGAATTAAGAGAAGCA delELREA 73 % (2467)
BM 72 40 L858R L858R c.2573 T > G L858R 61 % (554)
BM 75 48 G719X G719X c.2155G > A, G719S, 24 % (63),
     c.2327G > A R776H, 58 % (28),
BM 76 2 DEL 19 none Not detected by automatic analysis   
BM 82 24 L858R L858R c.2573 T > G L858R 25 % (129)
BM 101 4 DEL 19 DEL 19 c.2185G > A G729R, 6 % (10),
     c.2235_2249delGGAATTAAGAGAAGC delKELREA, 26 % (48),
     c.2260A > C K754Q, 27 % (50),
     c.2279 T > C L760P, 6 % (11),
BM 145 14 DEL 19 DEL 19 Not detected by automatic analysis   
BM 150 56 DEL 19 DEL 19 Not detected by automatic analysis   
BM 157 72 L858R L858R c.2573 T > G, L858R 74 % (792),
BM 159 3 L858R none Not detected by automatic analysis   
     c.2125G > A, E709K, 7 % (36),
BM 160 5 L858R none Not detected by automatic analysis   
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BMC Pulmonary Medicine

ISSN: 1471-2466

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