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Table 3 NGS run metrics for false negative results samples

From: Comparison of next generation sequencing, SNaPshot assay and real-time polymerase chain reaction for lung adenocarcinoma EGFR mutation assessment

Id Mutation Mean read depth Hotspot coverage Hotspot relative coverage Variant frequency (%) Variant coverage Tumor cell content (%) DNA concentration (ng/μl)
BM 65 L858R 1039 435 0.41 4.6 20 10 5.05
BM 76 DEL19 891 1028 1.15 2.9 29 2 22.9
BM 145 DEL19 36 2 0.05 100 2 14 4.91
BM 159 L858R 480 322 0.66 2.2 7 3 11.9
BM 160 L858R 436 299 0.68 4.4 13 5 0.22