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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Eosinophils enhance WNT-5a and TGF-β1 genes expression in airway smooth muscle cells and promote their proliferation by increased extracellular matrix proteins production in asthma

Fig. 1

Combined culture of airway smooth muscle cells (ASMC) and eosinophils. a ASMC incubated alone on the first day of cultivation. b Combined culture of confluent ASMC and eosinophils from asthmatic subjects after 30 min of incubation, demonstrating the interaction of ASMC with eosinophils. c Combined culture of confluent ASMC with eosinophils from asthmatic individuals after 30 min of incubation and three washes with warm phosphate-buffered saline (PBS), demonstrating the proportion of eosinophils stably adhering to viable ASMC. d ASMC after 4 min of incubation with warm PBS containing 1 mM EDTA, demonstrating effective separation of combined cultures. Sufficient confluency was considered to be 75 %, equivalent to 2 × 105 ASMC. Eosinophils from asthmatic subjects were seeded at 2 × 105 cells per well. Magnification in panels A and D: wide-field eyepiece, ×10; objective, ×10. Magnification in panels B and C: wide-field eyepiece, ×20; objective, ×10

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