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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Eosinophils enhance WNT-5a and TGF-β1 genes expression in airway smooth muscle cells and promote their proliferation by increased extracellular matrix proteins production in asthma

Fig. 2

Combined culture of airway smooth muscle cells (ASMC) and eosinophils from asthmatic and healthy individuals. a ASMC incubated with eosinophils from asthmatic subjects. b Combined culture of ASMC and eosinophils from healthy subjects. c Combined culture of ASMC and eosinophils from asthmatic subjects after 30 min of incubation and three washes with warm phosphate-buffered saline (PBS). d ASMC after 30 min of co-culture with eosinophils from healthy subjects and three washes with warm PBS. The number of eosinophils (0.5 × 105) and ASMC (2 × 105) per well is the same in all panels. A: absorbance at 490 nm, representing eosinophil peroxidase activity in ASMC and eosinophil co-cultures. A(control): absorbance in control ASMC cultured without eosinophils. Sufficient confluency was considered to be 75 %, equivalent to 2 × 105 cells. Magnification: wide-field eyepiece, ×10; objective, ×10

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BMC Pulmonary Medicine

ISSN: 1471-2466

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