Table 1 Circulating peripheral blood mononuclear cells quantified by flow cytometry
Tube | Cell subset | Immunophenotypes | |
|---|---|---|---|
Paternal cells | Derivative cells | ||
1 | Natural killer T cells | CD45+/CD56+ | CD3+ |
Natural killer cells | CD3− | ||
B lymphocytes | CD45+/CD16low/CD14− | CD19+ | |
T lymphocytes | CD3+ | ||
2 | T helper lymphocytes | CD3+ | CD4+/CD8− |
Cytotoxic T lymphocytes | CD4−/CD8+ | ||
TCRγδ lymphocytes | CD4−/CD8−/TCRγδ+ | ||
3 | TH1 lymphocytes | CD3+/CD4+ | CXCR3+/CCR4−/CCR6− |
TH1/TH17 lymphocytes | CXCR3+/CCR4−/CCR6+ | ||
TH17 lymphocytes | CXCR3−/CCR4+/CCR6+ | ||
TH2 lymphocytes | CXCR3−/CCR4+/CCR6− | ||
4 | Regulatory T lymphocytes | CD3+/CD4+ | CD25high/CD127low |