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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)

Fig. 4

Time course of water pipe condensate (WPC) on the uptake of FITC-BCG. FITC-BCG uptake by A549 cells was increased in a time-dependent manner compared to PBS-treated cells. Uptake was increased 1.3- and 1.4-fold after 72 and 96 h exposure to WPC, respectively while no effect on uptake was seen after 24 and 48 h on cells. a PBS control; b 24 h; c 48 h; d 72 h; and e 96 h exposure. Data are presented of three independent experiments. The data are presented graphically in (f) which shows the percentages of FITC-BCG positive cells at different time points in response to WPC compared to PBS exposure. PBS exposure had no effect on uptake and time course data are presented relative to PBS control. All dot and bars plots results are presented as mean ± SD of the three independent experiments each repeated in triplicate. *p < 0.05; **p < 0.01 versus control was calculated

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