Table 1 Comparison of FISH, IHC, qRT-PCR, NGS and NanoString regarding costs, procedural difficulty and resolution

Costs

Overal reagents costs

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*

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Hardware & software costs

**

***

**

*

*

Procedural difficulty

RNA input required

NA

NA

*

***

**

Poor quality

samples

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*

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Hands on time

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**

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***

Run time and results analysis

**

**

**

*

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Interpretation of results

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**

***

**

Throughput

*

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**

***

**/***

Resolution

Accuracy at low concentrations

NA

NA

**

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***

Quantitative precision

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**

***

***

Variants detection

*

*

**

***

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