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Table 1 Comparison of FISH, IHC, qRT-PCR, NGS and NanoString regarding costs, procedural difficulty and resolution

From: Detection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology

   FISH IHC qRT-PCR NGS NanoString
Costs Overal reagents costs */** *** **/*** * **/***
  Hardware & software costs ** *** ** * *
Procedural difficulty RNA input required NA NA * *** **
  Poor quality
samples
*/** **/*** * */** **/***
  Hands on time */** */** ** */** ***
  Run time and results analysis ** ** ** * **/***
  Interpretation of results */** */** ** *** **
  Throughput * */** ** *** **/***
Resolution Accuracy at low concentrations NA NA ** */** ***
  Quantitative precision */** */** ** *** ***
  Variants detection * * ** *** **/***
  1. *-worse option; **-moderate option; *** - best option: NA Not applicable