Table 3 Technical validation data of the DCN-CS ELISA assay

Detection range (LLOD-ULOD)

1.2–345.3 ng/mL

Lower limit of quantification (LLOQ)

5.3 ng/mL

Intra-assay variation

3%

Inter-assay variation

13%

Dilution of serum samples

1:4

Dilution recovery (1:4 pre-dilution)^a

100% (82–113%)

Freeze/thaw recovery (4 cycles)^a

94% (90–97%)

Analyte stability up to 24 h, 4 °C^a

87% (86–90%)

Interference Lipids, low/high

107%/86%

Interference Biotin, low/high

100%/100%

Interference Hemoglobin, low/high

100%/100%