Fig. 1

Validation of Arg1 ablation in lung of female Arg1 ^fl/fl mice. a Lungs of PBS/OVA- and OVA/OVA-treated female control mice, respectively (top row), and of OVA/OVA-treated female Arg1-KO^Tie2 and Arg1-KO^LysM mice, respectively (bottom row). Note the complete absence of ARG1-positive macrophages in Arg1-KO^Tie2 mice and the reduction in Arg1-KO^LysM mice. b Reduction of ARG1 protein content in the lungs of OVA/OVA-treated Arg1-KO^Tie2 and Arg1-KO^LysM mice (white bars) compared to the corresponding Arg1-Con^LysM or Arg1-Con^Tie2 mice (black bars). Means ± SEM (n = 8 mice per group). c Number of ARG1-positive cells per mm² lung tissue. Cells were counted near bronchioles and arteries (black bars) and in the lung parenchyma (white bars). Means ± SEM are shown (n = 8 mice per group). d Detail of ARG1 protein content in OVA/OVA-treated control lung. The absence of ARG1 in arterial endothelium is noteworthy. Abbreviations: A = artery, and M = ARG1-positive macrophages. Significance symbols: *: P < 0.001 OVA/OVA vs. PBS/OVA Arg1-Con; #: P < 0.001 OVA/OVA vs. PBS/OVA Arg1-KO^Lysm; ‡: P < 0.001 OVA/OVA Arg1-KO^Lysm or Arg1-KO^Tie2 vs. OVA/OVA Arg1-Con