Tobacco-smoking induced GPR15-expressing T cells in blood do not indicate pulmonary damage

BMC Pulmonary Medicine

Table 2 Smoking-dependent proportion of GPR15-expressing cells among different lymphocytes and cg05575921 methylation in granulocytes of blood from patients with lung disease

	GPR15+ cells among blood lymphocytes						CpG methylation
	Lymphocyte	T cell	T-helper	T-cytotoxic	B cell	NK cell	Granulocyte
	Lymphocyte	[CD3+]	[CD4+]	[CD8+]	[CD19+]	[CD15/56+]	cg05575921
A) Comparison of individuals for smoking
mean [confidence interval][%]
Smoking
never [n, 10]	3.9	4.5	5.9	1.9	8.9	3.3	75.9
never [n, 10]	[2.8-5.0]	[3.3-5.6]	[4.5-7.4]	[0.9-2.9]	[−1.7-19.4]	[−0.1-6.7]	[71.9-79.9]
former [n, 21]	11.7	16.0	18.5	7	4.9	1.9	53.5
former [n, 21]	[9.5-13.8]	[13.3-18.2]	[16.1-20.9]	[4.1-9.8]	[1.9-7.9]	[0.9-2.8]	[48.5-58.6]
current [n, 11]	21.7	31,4	35	16	4	3.6	33.7
current [n, 11]	[16.1-27.4]	[24.0-38.9]	[26.3-43.7]	[12.0-20.0]	[2.2-5.9]	[2.0-5.1]	[23.9-43.5]
p-value ^a
Smoking	<0.001	<0.001	<0.001	<0.001	0.953	0.026	<0.001
never vs former	0.004	0.001	0.002	0.006	1.000	1.000	0.01
never vs current	<0.001	<0.001	<0.001	<0.001	1.000	0.132	<0.001
former vs current	0.019	0.010	0.010	0.008	1.000	0.027	0.02
B) Use of cellular parameter as biomarker to predict never smoking status
cut-off [%]^b	6.9	7.6	10.0	4.8	38.4	12.9	67.2
Specificity	0.85	1.00	1.00	0.73	0.00	0.00	0.91
Sensitivity	1.00	1.00	1.00	0.90	0.90	1.00	1.00

ISSN: 1471-2466