Fig. 3

siRNA-TERRA decreased the H₂O₂-treated cell apoptosis. a Flow cytometry was used to analyze the cell apoptosis: a. normal A549 cells; b. A549 cells treated with H₂O₂ alone, c. A549 cells transfected with siRNA-negative control for 48 h and co-treated with 120 μM of H₂O₂ for 12 h, d. A549 cells transfected with siRNA-TERRA1 for 48 h and co-treated with 120 μM of H₂O₂ for 12 h, and e. A549 cells transfected with siRNA-TERRA2 for 48 h and co-treated with 120 μM of H₂O₂ for 12 h. b Cell apoptotic rates were quantitatively analyzed. siRNA-TERRA can decrease the apoptosis of A549 compared with H₂O₂-treated group. c The apoptosis of MLE-12 cell was tested by flow cytometry. a. normal MLE-12 cells, b. MLE-12 cells treated with H₂O₂ alone, c. MLE-12 cells transfected with siRNA-negative control for 48 h and co-treated with 60 μM of H₂O₂ for 12 h, d. MLE-12 cells transfected with siRNA-TERRA1 for 48 h and co-treated with 60 μM of H₂O₂ for 12 h, and e. MLE-12 cells transfected with siRNA-TERRA2 for 48 h and co-treated with 60 μM of H₂O₂ for 12 h. d Cell apoptotic rates were quantitatively analyzed. siRNA-TERRA can decreased the apoptosis of MLE-12 compared with H₂O₂-treated group. Each bar represents mean ± SD, n = 6. ^** P < 0.01 compared with the normal control group. ^# P < 0.05 and ^## P < 0.01 compared with the H₂O₂+ siRNA-TERRA negative control group