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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: TLR4-NOX2 axis regulates the phagocytosis and killing of Mycobacterium tuberculosis by macrophages

Fig. 1

LPS upregulates TLR4 and NOX2 expression and ROS levels in THP-1 cells. a THP-1 cells or THP-1(A) cells were treated with or without LPS, incubated with TLR4-PE, and bound TLR4-PE levels were measured using flow cytometry as an indicator of TLR4 expression. Additionally, some THP-1(A) cells were infected with M. tuberculosis. Histograms of the results, with percentage of cells expressing high TLR4 indicated. b Bar graph of percentages from a. (*; P < 0.05 compared to its control). c Western blot of NOX2 expression in THP-1(A) cells with or without pretreatment with LPS, with or without subsequent M. tuberculosis infection. d Bar graph of band intensities from c. (*; P < 0.05). e Intracellular ROS levels were measured using flow cytometry. f Bar graph of ROS levels from e. [*; P < 0.05 compared to LPS(−)]

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