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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: TLR4-NOX2 axis regulates the phagocytosis and killing of Mycobacterium tuberculosis by macrophages

Fig. 2

Blocking TLR4 and NOX2 inhibits the phagocytosis of M. tuberculosis by THP-1 cells. a THP-1(A) cells were incubated with M. tuberculosis-FITC for various time periods (1–6 h) at 0 °C or 37 °C. THP-1(A) cells were isolated and identified by flow cytometry (top left), and amount of M. tuberculosis-FITC in those cells was also measured by flow cytometry (bottom left). Histogram of results (middle) with percentage of cells containing high levels of FITC indicated for the 37 °C group. Bar graph of percentages (right). (*; P < 0.05 compared to t = 1 h). b THP-1(A) cells treated without LPS, with LPS, or with LPS in combination with DPI (10 μM), DPI (20 μM), or HTA125 pretreatment were infected with M. tuberculosis-FITC, isolated, and analyzed by flow cytometry. The proportion of cells containing high levels of FITC is indicated (top). Bar graph of percentages (bottom). (*; P < 0.05). c THP-1(A) cells were incubated with various concentrations of LPS (0–1000 ng/mL) and subsequently infected with various concentrations of M. tuberculosis-FITC (1:1–1:100). The proportion of cells containing high levels of FITC was measured as in b. Bar graph of the results. (*; P < 0.05 compared to lowest concentration of LPS). (FSC; forward scatter, SSC; side scatter)

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