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Fig. 7 | BMC Pulmonary Medicine

Fig. 7

From: NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD

Fig. 7

A dynamic expression of NOX4, α-SMA, Collagen I induced by TGFβ1 in human primary artery smooth muscle cells (HPASMCs). HPASMCs were cultured in the presence of TGFβ1 at various concentrations (0–10 ng/mL) for different time periods (0–48 h) before the cell lysates were used for analysis. a Representative images of immunoblots of NOX4, α-SMA and Collagen I in PASMCs exposed to 2.0 ng/ml of TGFβ1 for indicated time points (0–48 h); (b) relative expresions of NOX4, α-SMA and Collagen I determined by a densometric assay of immunoblots of (a). c Immunoblots of NOX4, α-SMA and Collagen I in PASMCs exposed to indicated concentrations of TGFβ1 (0-10 ng/mL) for 24 h; (d) relative expresions of NOX4, α-SMA and Collagen I determined by a densometric assay of immunoblots (c). Bar graphs represent mean ± SEM from three independent experiments. Compared with the TGFβ1 untreated group, *: P < 0.05 (N = 3)

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