Fig. 3
From: Regional heterogeneity in response of airway epithelial cells to cigarette smoke
Effects of CSE exposure on COX-2 expression in SAECs and NHBEs. a COX-2 mRNA and (b) protein expression, with β-actin used as an internal and loading control, respectively. Band size is indicated on the right. Cells were exposed to 2.5% CSE for 24 h or not (controls). c Time-course of COX-2 mRNA expression in SAECs and NHBEs following 2.5% CSE exposure. β-Actin was used as an internal control. d Time course of COX-2 protein expression in SAECs and NHBEs following 2.5% CSE exposure. β-Actin was used as a loading control. Band size is indicated on the right. Values are shown as the mean ± SEM. *p < 0.05; **p < 0.01; *** p < 0.001 compared with NHBEs in (c) and (d). Three batches each of SAECs and NHBEs were evaluated on three separate occasions in all experiments. CSE, cigarette smoke extract; SAECs, small airway epithelial cells; NHBEs, normal human bronchial epithelial cells; COX-2, cyclooxygenase-2