Fig. 1

Effect of hyperoxia on interleukin (IL)-3 and IL-3-specific receptor α chain (IL-3Rα). C57BL/6 mice were challenged with room air (control) or hyperoxia for 72 h and pulmonary protein of IL-3 and IL-3Rα were assessed by western blotting, and IL-3 levels in plasma was measured by enzyme linked immunosorbent assay (ELISA) (a). Neutrophils in bronchoalveolar lavage fluids (BALF) (b) and circulation (c), protein in BALF (d), lung edema (e), and lung injury score (f) were assessed 72 h after hyperoxia or room air challenge. Data represent assessments in a minimum of n = 5 mice. ^#P < 0.05 vs. control; ^*P < 0.05 vs. sham+wild type (WT) group; ^† P < 0.05 vs. hyperoxia +WT group. IL-3^−/−, IL-3 gene disrupted mice