Fig. 6
From: Role of TLR4-p38 MAPK-Hsp27 signal pathway in LPS-induced pulmonary epithelial hyperpermeability
Role of p38 inhibitor SB203580 in LPS-induced A549 hyperpermeability. A549 was pretreated with SB203580 followed by LPS exposure. (a) TER value and (b) permeability coefficient for dextran (Pd) in dextran trans-epithelial flux were measured; (c) P-p38 and P-Hsp27 were detected by western blotting; (d) The image of rhodamine-phalloidin stained F-actin was obtained through a laser confocal microscopy; (e) Fluorescence intensity of F-actin was qualified; (f) The image of rhodamine-phalloidin stained F-actin was obtained through a laser confocal microscopy by using primary mice pulmonary epithelial cells. n = 3, *P < 0.05 versus control, #P < 0.05 versus LPS