Fig. 2

LPS induced inflammatory response in C57BL/6 mice. Proinflammatory cytokines IL-6 (a) and TNF-α (c) as well as the chemokine KC (b) were quantified in BALF supernatants on day 1, 4 and 10 after ARDS induction by ELISA. d Total cell numbers in BALF of LPS-treated animals and saline-instilled controls were calculated by FACS analysis. e–j Several cell subpopulations in ARDS mice were differentiated by specific antibodies, neutrophil granulocytes (e), T cell subpopulations (f–i) and CD4⁺/CD8⁺ ratio (F). Data are shown as means ± SEM. a–j Controls n = 4, ARDS groups n = 6. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001