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Fig. 6 | BMC Pulmonary Medicine

Fig. 6

From: WISP1 mediates lung injury following hepatic ischemia reperfusion dependent on TLR4 in mice

Fig. 6

WISP1 Contributes to Lung Injury in Hepatic IRI Dependent on TLR4. WT and TLR4−/− mice in sham and IRI group undergoing ischemia for 60 min and reperfusion for 6 h were treated intraperitoneally with either control IgG antibody or anti-WISP1 antibody (6 μg/g) 1 h before ischemia and at the onset of reperfusion (n = 6 respectively). In another experiment, WT and TLR4−/− mice were treated intraperitoneally with either sterile PBS or rWISP1 (1 μg/g) at the onset of reperfusion (n = 6 respectively). Western blot and qRT-PCR analysis (a) of WISP1 in the lung tissue from all groups were performed. W/D weight ratio (b), EBA permeability (c) for mice treated with control IgG antibody or anti-WISP1 antibody were analyzed. W/D weight ratio (d) and EBA permeability (e) for mice administered with sterile PBS or rWISP1 were analyzed. a *p < 0.05 compared with sham group, #p < 0.05 compared with I/R(TLR4+/+) group, (b, c) *p < 0.05 compared with I/R (IgG) group, (d, e) *p < 0.05 compared with I/R (PBS) group, data are expressed as mean ± s.e.m. for all samples

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