Ligustrazin increases lung cell autophagy and ameliorates paraquat-induced pulmonary fibrosis by inhibiting PI3K/Akt/mTOR and hedgehog signalling via increasing miR-193a expression

Table 1 Primer sequences for qRT-PCR

U6	F-5’-GTCGTATCCAGTGCAGGGTCCGAGGT-3’	174 bp
	R-5’-ATTCGCACTGGATACGACCAGACTC-3’
miR-193a	F-5’-CTCGCTTCGGCAGCACA − 3’	158 bp
	R-5’-AACGCTTCACGAATTTGCGT − 3’
Akt mRNA	F-5’-TCACCTCTGAGACCGACACC-3’	121 bp
	R-5’-ACTGGCTGAGTAGGAGAACTGG-3’
Beclin1 mRNA	F-5’-ATGCAGGTGAGCTTCGTGTG-3’	247 bp
	R-5’-CTGGGCTGTGGTAAGTAATGGA-3’
LC3-IImRNA	F-5’-AAACGCATTTGCCATCACA-3’	193 bp
	R-5’-GGACCTTCAGCAGTTTACAGTCAG-3’
mTOR mRNA	F-5’-AGAAACTGCACGTCAGCACCA-3’	217 bp
	R-5’-CCATTCCAGCCAGTCATCTTTG-3’
SHH mRNA	F-5’-CCAATTACAACCCCGACATC-3’	178 bp
	R-5’-GCATTTAACTTGTCTTTGCACCT-3’
Smo mRNA	F-5’-GGCTGCTGAGTGAGAAG-3’	194 bp
	R-5’-CTGGTTGAAGAAGTCGTAGAAG-3’
Gli-1 mRNA	F-5’-CCAAGCCAACTTTATGTCAGGG-3’	215 bp
	R-5’-AGCCCGCTTCTTTGTTAATTTGA-3’
TGF-β1mRNA	F-5′- TACCAGAAATACAGCAACA-3′	138 bp
	R-5’-TGACATCAAAAGATAACCA-3’
Collagen I mRNA	F-5′- TCTAGACATGTTCAGCTTTGTGGAC-3′	252 bp
	R-5′- TCTGTACGCAGGTGATTGGTG-3’
β-actin	F-5’-TACAACCTTCTTGCAGCTCC-3’	192 bp
	R-5’-ATCTTCATGAGGTAGTCTGTC-3’

ISSN: 1471-2466