Skip to main content
Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Caffeine is associated with improved alveolarization and angiogenesis in male mice following hyperoxia induced lung injury

Fig. 4

Assessment of vessel wall thickness at 12wks by α-smooth muscle actin immunohistochemistry of lung sections (100x: a/c/e/g; 400x: b/d/f/h; room air saline (RAS): A/B; room air caffeine (RAC): c/d; hyperoxia saline (HS): e/f; hyperoxia caffeine (HC): g/h). Scale: 200 μm (100x), 60 μm (400x). Thick arrows (400x) correspond to the same histologic location as thin arrows (100x) of the same group. Demonstrated vessels are of a 30-50 μm diameter in all the four groups. Pulmonary arterial vessel wall thickness was greater in the hyperoxia group suggestive of smooth muscle hypertrophy (i; *p < 0.001 vs. RAS; Fisher’s post-hoc test, ANOVA). Caffeine administration attenuated vessel wall thickness, especially in male mice at 12 weeks of age (**p < 0.0001 vs HS group, Fisher’s post-hoc test, ANOVA; i), suggesting it may have beneficial effects on the pulmonary vasculature (Open box plots – RA groups; shaded box plots – hyperoxia groups) (n = ten mice/group)

Back to article page
\