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Table 2 Protein expression of selected angiogenic proteins and cytokines in the lung measured by enzyme immunoassay (EIA) in newborn mice exposed to hyperoxia (95%O2) or room air and administered caffeine or saline

From: Caffeine is associated with improved alveolarization and angiogenesis in male mice following hyperoxia induced lung injury

Protein Room Air Saline Group (RAS) Room Air Caffeine Group (RAC) Hyperoxia Saline Group (HS) Hyperoxia Caffeine Group (HC)
HIF & Angiogenic Proteins (ng or pg/mg protein)
 Hif-1α (pg) 230 ± 25 215 ± 41 250 ± 40 253 ± 46
 Hif-2α (ng) 0.232 ± 0.058 0.117 ± 0.008 0.274 ± 0.055 0.464 ± 0.159*
 Hif-3α (pg) 6.96 ± 1.78 5.99 ± 0.51 7.60 ± 1.31 6.05 ± 0.78
 VEGF (pg) 55.5 ± 16.1 64.6 ± 7.1 177.5 ± 52.8** 92.6 ± 16.4
 Flt-1 (pg) 2297 ± 1026 1714 ± 85 3364 ± 1590 5866 ± 3086*
 Ang-1 (ng) 522 ± 192 664 ± 72 258 ± 70† 455 ± 70
Cytokines
 TNF-α (pg/μg protein) 0.350 ± 0.01 0.040 ± 0.01 0.043 ± 0.02 0.045 ± 0.01
 INF-γ (pg/μg protein) 0.098 ± 0.04 0.109 ± 0.03 0.118 ± 0.04 0.134 ± 0.02
 IL-6 (pg/μg protein) 0.065 ± 0.02 0.062 ± 0.01 0.081 ± 0.04 0.066 ± 0.01
  1. HIF & angiogenic proteins are expressed in ng or pg/mg protein of the lung (n = 8 pups in each group)
  2. Values expressed as mean ± SD. * p < 0.05 vs RAC & RAS groups, **p < 0.05 vs RAS, RAC & HC groups, †p < 0.05 vs RAS, RAC & HC groups; (Fisher’s post-hoc test, ANOVA). HIF-1α - Hypoxia-Inducible Factor-1α; HIF-2α - Hypoxia-Inducible Factor-2α; HIF-3α - Hypoxia-Inducible Factor-3α; VEGF - Vascular Endothelial Growth Factor; Flt-1: VEGF Receptor 1; Ang-1: Angiopoietin-1; TNF-α: tumor necrosis factor alpha; INF-γ: interferon gamma; IL-6: interleukin-6. HIF & angiogenic proteins are expressed as units/mg lung protein
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