|
Protein
|
Room Air Saline Group (RAS)
|
Room Air Caffeine Group (RAC)
|
Hyperoxia Saline Group (HS)
|
Hyperoxia Caffeine Group (HC)
|
|---|
|
HIF & Angiogenic Proteins (ng or pg/mg protein)
|
|
Hif-1α (pg)
|
230 ± 25
|
215 ± 41
|
250 ± 40
|
253 ± 46
|
|
Hif-2α (ng)
|
0.232 ± 0.058
|
0.117 ± 0.008
|
0.274 ± 0.055
|
0.464 ± 0.159*
|
|
Hif-3α (pg)
|
6.96 ± 1.78
|
5.99 ± 0.51
|
7.60 ± 1.31
|
6.05 ± 0.78
|
|
VEGF (pg)
|
55.5 ± 16.1
|
64.6 ± 7.1
|
177.5 ± 52.8**
|
92.6 ± 16.4
|
|
Flt-1 (pg)
|
2297 ± 1026
|
1714 ± 85
|
3364 ± 1590
|
5866 ± 3086*
|
|
Ang-1 (ng)
|
522 ± 192
|
664 ± 72
|
258 ± 70†
|
455 ± 70
|
|
Cytokines
|
|
TNF-α (pg/μg protein)
|
0.350 ± 0.01
|
0.040 ± 0.01
|
0.043 ± 0.02
|
0.045 ± 0.01
|
|
INF-γ (pg/μg protein)
|
0.098 ± 0.04
|
0.109 ± 0.03
|
0.118 ± 0.04
|
0.134 ± 0.02
|
|
IL-6 (pg/μg protein)
|
0.065 ± 0.02
|
0.062 ± 0.01
|
0.081 ± 0.04
|
0.066 ± 0.01
|
- HIF & angiogenic proteins are expressed in ng or pg/mg protein of the lung (n = 8 pups in each group)
- Values expressed as mean ± SD. * p < 0.05 vs RAC & RAS groups, **p < 0.05 vs RAS, RAC & HC groups, †p < 0.05 vs RAS, RAC & HC groups; (Fisher’s post-hoc test, ANOVA). HIF-1α - Hypoxia-Inducible Factor-1α; HIF-2α - Hypoxia-Inducible Factor-2α; HIF-3α - Hypoxia-Inducible Factor-3α; VEGF - Vascular Endothelial Growth Factor; Flt-1: VEGF Receptor 1; Ang-1: Angiopoietin-1; TNF-α: tumor necrosis factor alpha; INF-γ: interferon gamma; IL-6: interleukin-6. HIF & angiogenic proteins are expressed as units/mg lung protein
