Fig. 2

DNA-PKcs does not co-localize with hypomethylated DNA in normal and IPF lung fibroblasts. a-p Normal and IPF lung fibroblasts were treated with 1 μM biotinylated-hypomethylated CpG DNA (biotin-CpG) for 24 h and then fixed and stained with Alexa-flour 594-conjugated-strepdavidin and anti-DNA-PKcs followed by Alexa-flour 488 conjugated secondary antibodies. As a control, untreated cells were stained with Alexa-flour 594-conjugated-strepdavidin and IgG control antibodies. Shown are representative images acquired at 200x magnification showing the localization of DNA-PKcs (a, e, i & m, green), biotin-CpG (b, f, j & n, red) or DAPI stained nuclei (c, g, k & o, blue) in normal (a-h) and IPF (i-p) lung fibroblasts. Composite images are shown in panels d, h, l & p for normal and IPF lung fibroblasts, respectively. n = 2 normal and n = 2 IPF. Scale bars = 50 μm