Fig. 6

Abundance of CgA expression in SSEA4⁺ progenitor cells and IPF lung tissues. a SSEA4⁺ cell RNAseq datasets (GSE103488 [9]) were mined for the expression of various lineage transcripts. Shown are the average FPKM values from 2 Normal-, 4 slow- and 4 rapid-SSEA4⁺ cells. b-c Normal and IPF stromal cultures were permeabilized and stained SSEA4 and CgA followed by flow cytometric analysis. Representative flow cytometric contour plots derived from 3 normal (b) and 2 IPF (c) stromal cultures stained and gated for SSEA4 (left) showing the expression of CgA protein in the gated cells (right). d Normal or IPF lung explants were stained for CgA and SSEA4 followed by fluorescently conjugated secondary antibody and fluorescent microscopy analysis. Representative images from Normal (top) and two IPF patients (bottom two rows) are shown stained with CgA (left) and SSEA4 (middle) and the merged composite (right) acquired at 200x magnification. e-i Normal (e), slow- (f), rapid- (g), IPF biopsies or explants (h-i) were histologically stained for CgA. Representative images were taken from 3 normal, 4 slow- & 5 rapid-IPF biopsies and 12 IPF explants. IgG control staining are shown in inlayed images