Skip to main content
Fig. 7 | BMC Pulmonary Medicine

Fig. 7

From: DNA-PKcs modulates progenitor cell proliferation and fibroblast senescence in idiopathic pulmonary fibrosis

Fig. 7

DNA-PKcs inhibition in IPF explant cell-humanized NSG mice promoted expansion of SSEA4+ S100A4+ cells. a IPF lung explant cells were intravenously administered into NSG mice. Thirty-five days after administration, mice were treated interperitoneally with saline or 10 mg/kg of Nu7441, twice a week for 4 weeks. After a total of 63 days, mice were sacrificed, and their lung tissues were collected for analysis. Shown are the (b) average hydroxyproline concentration in the lungs of mice and (c) representative western blot of lung lysates from saline and Nu7441 treated NSG mice for alpha-SMA (αSMA, top) and GAPDH (bottom) proteins and densitometric ratios of αSMA normalized to GAPDH proteins from saline and Nu7441 treated mice. Data shown are mean ± s.e.m.; n = 4–5/group. One-way ANOVA followed by Tukey’s multiple comparisons test. (d) Masson’s trichrome staining of naive and IPF lung explant cell–challenged NSG mouse lungs treated with saline or Nu7441. e RNA was extracted from saline or Nu7441 treated NSG mouse lungs and qPCR analysis was performed for various human transcripts. Shown is the average expression of the indicated transcripts in Nu7441 relative to saline treated NSG lungs. Data shown are mean ± s.e.m.; n = 4/group. f One out of five mice receiving Nu7441 developed a mass in the thoracic cavity. g-k Shown are representative images acquired at 50x magnification of the tissue mass stained for human-S100A4 (g), SSEA4 (h), human-CgA (i), human-CCR10 (j), Ki-67 (k) or IgG control (inlay) antibodies

Back to article page