Fig. 5

PRDX4 expression in mice. a) Immunohistochemical staining of murine lung for detecting PRDX4. PRDX4-positive cells, indicating PRDX4 expression, were stained brown. b) Double immunohistochemical staining of murine lung. Human PRDX4 (cytoplasm is green stained) and Mac-2 (cytoplasm is red stained), human PRDX4 (cytoplasm is green stained), and TTF-1 (nucleus is red stained) are expressed in same cells in Tg-saline. Human PRDX4 (cytoplasm is green stained) and Mac-2 (cytoplasm is red stained), human PRDX4 (cytoplasm is green stained) and TTF-1 (nucleus is red stained), human PRDX4 (cytoplasm is green stained) and α-SMA (cytoplasm is red stained) are expressed in the same cells in Tg-BLM. c and d) An ELISA for assessing the BALF and serum human PRDX4 protein levels. The BALF and serum human PRDX4 protein levels were markedly higher in Tg-BLM than in WT-BLM. The serum human PRDX4 protein levels in Tg-baseline and Tg-saline mice were significantly higher than in the WT mice. e) The human PRDX4 mRNA levels in murine lung homogenates. The human PRDX4 mRNA levels were markedly higher in Tg-baseline, Tg-saline and Tg-BLM than in WT mice