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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Knockdown of circ-ABCB10 promotes sensitivity of lung cancer cells to cisplatin via miR-556-3p/AK4 axis

Fig. 1

Circular RNA circ-ABCB10 is highly expressed and downregulation of it represses lung cancer progression and improves the sensitivity of lung cancer cells to cisplatin. a The expression of circ-ABCB10 in lung cells (H125, H1299, NCI-H292 and A549) and normal human bronchus epithelium cells (HBE) was detected via RT-qPCR. b Nucleic acid gel electrophoresis analysis revealed that divergent primers could produce the circular isoform of ABCB10 with cDNA but not with gDNA. GAPDH was used as a negative control. c The stability of circ-ABCB10 and ABCB10 mRNA was detected by RT-qPCR in A549 and NCI-H292 cells after treatment of ActD. d RT-qPCR assay was conducted to determine the abundance of circ-ABCB10 and linear ABCB10 mRNA in A549 and NCI-H292 cells treated with Rnase R (normalized to mock treatment). e The expression of circ-ABCB10 in A549 and NCI-H292 cells treated with different chemotherapy agents was determined via RT-qPCR analysis. f The efficiency of circ-ABCB10 knockdown in A549 and NCI-H292 cells was detected by RT-qPCR. g-h After treatment with or without cisplatin, the proliferation ability of transfected cells was measured by CCK-8 and colony formation assays. i TUNEL analysis of cell apoptosis ability in transfected cells with or without cisplatin treatment. j Transwell analysis of cell migration capability in transfected cells with or without cisplatin treatment. *P < 0.05, **P < 0.01

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