Fig. 4

Effects of Cx43 overexpression and Gap19 treatment on apoptosis and ROS production in HUVECs. After Cx43 overexpression with or without 100 μM Gap19 for 24 h, apoptosis and ROS production were assessed, respectively, by Annexin V-FITC/PI and DCFH-DA using flow cytometry. Cx43 overexpression and Gap19 treatment showed decreased cell apoptosis (a, b) and ROS production (c, d) in HUVECs. Experiments were performed in 3 biological replicates and data are presented as mean ± SD. **P < 0.01 compared with control. ^##P < 0.01 compared with Cx43 overexpression