Fig. 1

Case 1 – Solitary pulmonary nodule in apical segment of right upper lobe. a: 2.23 cm solitary pulmonary nodule in apical segment (medial sub-segment) of right upper lobe. b: An eccentric rEBUS orientated lesion localized at RB1aii with no surrounding vessel. c: Placement of 1.1 mm flexible cryoprobe through GS into the target lesion at apical segment of right upper lobe under fluoroscopic guidance. d: Retrieved cryobiopsy specimen upon en bloc removal of bronchoscope-GS-cryoprobe. e: Gross appearance of fragmented forceps biopsy in comparison with cryobiopsy specimen. f: forceps biopsy revealed fragmented bronchial tissue with rare cluster of atypical cells (Hematoxylin & Eosin, × 400 magnification). g: Cryobiopsy revealed fragments of alveolated parenchymal infiltrated with tumour cells in glandular pattern, exhibiting enlarged and pleomorphic nuclei (Hematoxylin & Eosin, × 100 magnification). Immunohistochemistry shows diffuse and strong positivity to TTF-1, consistent with adenocarcinoma of lung