Fig. 2

The specific role of miR-4677-3p in LAD. (a) Potential binding targets for LINC02418 were predicted by bioinformatics analysis. (b) Relative expression of miR-4677-3p influenced by LINC02418 knockdown was detected via RT-qPCR. (c) Relative expression of miR-4677-3p in LAD cell lines and normal pulmonary epithelial cell line was examined by RT-qPCR. (d) The transfection efficiency of miR-4677-3p mimics was checked by RT-qPCR. (e) CCK-8 assay was carried out to test cell proliferation when A549 and SPC-A1 cells were treated with miR-4677-3p mimics. (f) Colony formation assay was used to detect cell proliferation in miR-4677-3p mimics-transfected A549 and SPC-A1 cells (g) Transwell assay was performed to examine the migration of A549 and SPC-A1 cells treated with miR-4677-3p mimics. (h) Western blot was conducted to access the level of migration-related proteins in A549 and SPC-A1 cells when overexpressing miR-4677-3p. ^**P < 0.01