Skip to main content
Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: LINC02418 promotes malignant behaviors in lung adenocarcinoma cells by sponging miR-4677-3p to upregulate KNL1 expression

Fig. 3

KNL1 is a downstream target gene of miR-4677-3p. a RNA22 and miRmap were used to research the potential target genes for miR-4677-3p. b The expression of the indicated mRNAs in miR-4677-3p mimic-transfected cells were tested using RT-qPCR. c The expression of KNL1 protein was detected by western blot when upregulating miR-4677-3p. d The mRNA and protein levels of KNL1 were examined by RT-qPCR and western blot analyses after downregulating LINC02418. e The enrichment of LINC02418, miR-4677-3p and KNL1 in RISC complex was determined by RIP assay. f The binding site between miR-4677-3p and LINC02418 was predicted by starBase (left). Luciferase reporter assay measured the luciferase activities of LINC02418-WT and LINC02418-Mut (right). g The binding site between miR-4677-3p and KNL1 was predicted by starBase (upper). Luciferase reporter assay measured the luciferase activities of KNL1-WT and KNL1-Mut. h RNA pull down assay examined the enrichment of LINC02418 and KNL1 in miR-4677-3p biotin probe group. miR-4677-3p no-biotin probe served as negative control. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page