Lung-protective ventilation suppresses systemic and hepatic vein levels of cell-free DNA in porcine experimental post-operative sepsis

Nyberg, Axel; Larsson, Alexander; Jylhävä, Juulia; Hurme, Mikko; Sperber, Jesper; Lipcsey, Miklós; Castegren, Markus

doi:10.1186/s12890-020-01239-y

Table 3 Levels of trans-organ differences in inflammatory cytokines and cell free DNA (cf-DNA)

From: Lung-protective ventilation suppresses systemic and hepatic vein levels of cell-free DNA in porcine experimental post-operative sepsis

		Group	0 h	3 h	5 h	p
TNF-a (ng x L⁻¹)	Transpulmonary	Prot-V	− 9222 ± 15,097	− 1293 ± 3383	− 284 ± 645	0.17
	Transpulmonary	Control	−13,878 ± 10,454	− 3819 ± 1832	− 477 ± 979
	Transhepatic	Prot-V	4919 ± 6573	1275 ± 3394	375 ± 664	0.91
	Transhepatic	Control	7531 ± 18,933	3638 ± 4823	− 459 ± 1589
	Transcerebral	Prot-V	2519 ± 6600	369 ± 1749	541 ± 648	0.24
	Transcerebral	Control	2154 ± 4868	1560 ± 6843	528 ± 977
	Transsplanchnic	Prot-V	5195 ± 11,969	−34 ± 2080	−27 ± 521	0.22
	Transsplanchnic	Control	4978 ± 13,845	672 ± 968	830 ± 1829
IL-6 (ng x L⁻¹)	Transpulmonary	Prot-V	33 ± 150	990 ± 470	493 ± 424	0.03*
	Transpulmonary	Control	118 ± 285	1753 ± 1263	2172 ± 632
	Transhepatic	Prot-V	17 ± 105	− 194 ± 258	−49 ± 220	0.19
	Transhepatic	Control	−21 ± 65	− 500 ± 282	− 281 ± 242
	Transcerebral	Prot-V	21 ± 183	−62 ± 762	−14 ± 499	0.43
	Transcerebral	Control	−16 ± 296	−84 ± 732	− 450 ± 557
	Transsplanchnic	Prot-V	−32 ± 174	− 711 ± 547	− 406 ± 484	0.14
	Transsplanchnic	Control	−126 ± 256	− 1100 ± 1264	− 1646 ± 2600
IL-10 (ng x L⁻¹)	Transpulmonary	Prot-V	−70 ± 81	−30 ± 17	−26 ± 15	0.18
	Transpulmonary	Control	−138 ± 101	−138 ± 39	−5 ± 100
	Transhepatic	Prot-V	−313 ± 298	−70 ± 63	−5 ± 32	0.31
	Transhepatic	Control	− 265 ± 221	−29 ± 109	90 ± 174
	Transcerebral	Prot-V	110 ± 95	32 ± 18	31 ± 20	0.18
	Transcerebral	Control	125 ± 83	73 ± 80	34 ± 133
	Transsplanchnic	Prot-V	428 ± 264	91 ± 60	27 ± 30	0.36
	Transsplanchnic	Control	344 ± 174	74 ± 68	61 ± 118
cf-DNA (μg x mL⁻¹)	Transpulmonary	Prot-V	0.02 ± 0.16	0.01 ± 0.14	0.03 ± 0.17	0.6
	Transpulmonary	Control	0.01 ± 0.03	−0.04 ± 0.08	−0.17 ± 0.39
	Transhepatic	Prot-V	0.03 ± 0.15	−0.01 ± 0.04	−0.01 ± 0.06	0.03*
	Transhepatic	Control	−0.01 ± 0.03	0.04 ± 0.09	0.12 ± 0.04
	Transcerebral	Prot-V	−0.01 ± 0.08	0.01 ± 0.03	0.01 ± 0.03	0.6
	Transcerebral	Control	−0.01 ± 0.03	0.03 ± 0.03	−0.01 ± 0.05
	Transsplanchnic	Prot-V	−0.02 ± 0.07	0.01 ± 0.04	0.02 ± 0.07	0.51
	Transsplanchnic	Control	−0.01 ± 0.02	0.01 ± 0.02	0.01 ± 0.05

Values are given as mean ± SD. P-values are results of group effect in the analysis of variance (ANOVA) for repeated measures. * denotes p < 0.05. TNF-α-tumor necrosis factor alpha, IL-6-interleukin 6, IL-10-interleukin 10. Transpulmonary: the difference in plasma levels between the artery and the hepatic vein, transhepatic: the difference between the hepatic vein and the portal vein, transsplanchnic: the difference between the portal vein and the artery, transcerebral: the difference between the jugular bulb and the artery

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ISSN: 1471-2466

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