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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: LncRNA PCGEM1 accelerates non-small cell lung cancer progression via sponging miR-433-3p to upregulate WTAP

Fig. 1

PCGEM1 is highly expressed in NSCLC cells and knockdown of it suppresses cell growth. a qRT-PCR was utilized to test the expression of PCGEM1 in NSCLC cell lines (A549, PC-9, NCI-H1299 and NCI-H1650) and the human lung bronchial epithelial cell line (BEAS-2B). b The interference efficiency of PCGEM1 in NCI-H1299 and NCI-H1650 cells was tested through qRT-PCR. c, d EdU and CCK-8 experiments were utilized to measure the cell proliferation when PCGEM1 was inhibited in NCI-H1299 and NCI-H1650 cells. e Flow cytometry analysis was implemented to evaluate cell apoptosis after PCGEM1 was knocked down in NCI-H1299 and NCI-H1650 cells. f, g Transwell assays were conducted to test cell migration and invasion after silencing of PCGEM1 in NCI-H1299 and NCI-H1650 cells. *P < 0.05, **P < 0.01

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BMC Pulmonary Medicine

ISSN: 1471-2466

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