Fig. 2

PCGEM1 serve as a miR-433-3p sponge in NSCLC. a The distribution of PCGEM1 in NCI-H1299 and NCI-H1650 cells was tested through subcellular fractionation assay. b The expressions of six miRNAs were tested through qRT-PCR in NSCLC cell lines (A549, PC-9, NCI-H1299 and NCI-H1650) and the human lung bronchial epithelial cell line (BEAS-2B). c The binding site of PCGEM1 and miR-433-3p was predicted by ENCORI database. d The utilization of qRT-PCR was utilized to test the overexpression efficiency of PCGEM1 in NCI-H1299 and NCI-H1650 cells. e The luciferase report experiments were utilized to confirm the binding situation of PCGEM1 and miR-433-3p in NCI-H1299 and NCI-H1650 cells. f RNA pull down assays were conducted to verify the interaction of PCGEM1 and miR-433-3p in NCI-H1299 and NCI-H1650 cells. ^**P < 0.01