Fig. 3

WTAP is a downstream target gene of miR-433-3p in NSCLC. a ENCORI was adopted to predict the possible mRNAs for miR-433-3p. b qRT-PCR was carried to screen out the mRNAs which could be declined by silenced PCGEM1 and overexpressed miR-433-3p. c qRT-PCR continued to test the expressions of WTAP and B4GALT3 in NSCLC cell lines (A549, PC-9, NCI-H1299 and NCI-H1650) and the human lung bronchial epithelial cell line (BEAS-2B). d The binding site of WTAP and miR-433-3p was predicted by ENCORI. e The binding relationship of WTAP and miR-433-3p was confirmed through luciferase report experiments. f RNA pull down assays were applied to verify the interaction of WTAP and miR-433-3p in NCI-H1299 and NCI-H1650 cells. g The knockdown efficiency of WTAP was tested by qRT-PCR in NCI-H1299 and NCI-H1650 cells. h, i Cell proliferation ability was evaluated through EdU and CCK-8 experiments after WTAP was knocked down in NCI-H1299 and NCI-H1650 cells. j Cell apoptosis was measured in NSCLC cells through flow cytometry when WTAP was silenced in NCI-H1299 and NCI-H1650 cells. k, l Transwell assays were conducted to estimate cell migration and invasion in cells which were transfected with silenced WTAP in NCI-H1299 and NCI-H1650 cells. ^*P < 0.05, ^**P < 0.01, n.s.: no significance