Fig. 2

LncRNA SNHG14 silencing enhanced the sensitivity of A549/DDP cell to DDP. a A549/DDP cells transfected with sh-NC or sh-SNHG14. qRT-PCR examined expression of lncRNA SNHG14A549/DDP. b After transfected with sh-NC and sh-SNHG14, the cell viability of A549/DDP cell was detected via CCK-8 under the treatment of DDP with different concentration (0, 2, 4, 6 and 8 μg/ml). c The cell proliferation of sh-NC and sh-SNHG14 treated A549/DDP cell was detected by colony formation experiment in the presence of DDP (1 μg/ml). d and e After 48 h of sh-SNHG14 transfection, the A549/DDP cell was treated with DDP (1 μg/ml) and then subjected for cell cycle and apoptosis detection using flow cytometry analysis. f Protein expression levels of Bcl-2, Bax and cleaved-caspase-3 were measured by western blot in lncRNA SNHG14 blocked A549/DDP cells treated with DDP (1 μg/ml)