Fig. 2
From: Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover
Strategy and results for comparative proteomics analysis of HBEs following CSE insult. a Experimental strategy and technical route for the quantitative proteomics analysis. In the LC-MS/MS analysis, two independent samples with or without CSE treatment were prepared. The proteins were digested with trypsin followed by co-immunoprecipitation using the SUMO-1 affinity columns. Each sample were undergone three parallel LC-MS/MS analyses to acquire quantitative proteomic data. b Venn diagram illustrating the overlap of identified proteins between control and CSE challenged HBEs. c A bar graphic figure showing 39 up-regulated proteins (2-fold changes) and 25 down-regulated proteins (2-fold changes). d A heatmap for the abundance of up-regulated (fold change ≥2) substrates. e A heatmap showing the abundance of the down-regulated (fold change ≤0.05) substrates