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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: IL-32 induces epithelial-mesenchymal transition by triggering endoplasmic reticulum stress in A549 cells

Fig. 3

Inhibition of EMT in A549 cells by IL-32 siRNA. a Transfection efficiency of A549 cells: A549 were cultured in vitro and inoculated onto six-well plates. When the cells reach confluence, Lipofectamine 3000 transfection reagent was used to transfect A549 cells with fluorescent plasmids containing IL-32 siRNA. Forty-eight hours after transfection, the IL-32 shRNA transfection was evaluated by observing the intensity of green fluorescent protein with an inverted fluorescence microscope. The IL-32 gene silencing efficiency was measured using RT-qPCR b and western blotting assay c, d. e The mRNA expression levels of EMT-related molecular markers after transfection with IL-32 siRNA. *P < 0.05 compared with the control group, **P < 0.05 compared with the TM group. f, g The protein expression levels of EMT-related molecular markers after transfection with IL-32 siRNA. *P < 0.05 compared with the control group, **P < 0.05 compared with the TM group

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