Fig. 2

Hypoxia reduce the megakaryocytes in lung and other hematopoietic tissues (flow cytometry). Area for R3 define the amount of positive cells (determined bythe IgG-control isotypes). a Representative dot-plots of the staining of cell suspensions from the lung; b Comparation of the proportion of megakaryocytes in lungs between the two groups. Each point represents the mean adjusted value of 3 replicates for each individual mouse(n = 8). P values were calculated using the Mann–Whitney U test. *P < 0.05; c Representative dot-plots of the staining of cell suspensions from the bone marrow; d Comparation of the proportion of megakaryocytes in the BW between the two groups. Each point represents the mean adjusted value of 3 replicates for each individual mouse(n = 8). P values were calculated using the t test. *P < 0.05; e Representative dot-plots of the staining of cell suspensions from the spleen; f Comparation of the proportion of megakaryocytes in the spleen between the two groups (n = 8). P values were calculated using the Mann–Whitney U test. *P < 0.05