Fig. 9

Expression and activity of key mitochondrial constituents in m. gastrocnemius in response to IT-LPS. mtDNA corrected for gDNA in m. gastrocnemius is depicted (b). CS (c) and HADH (d) activity in m. gastrocnemius are depicted. Protein expression of the following OXPHOS subunits in m. gastrocnemius is depicted; NDUFB8 of complex I (e), SDHB of complex II (f), UQCRC2 of complex III (g), and ATP5A of complex V (h). Representative immunoblots and a representative part of Ponceau S staining are shown, with adjusted contrast equally applied to whole photograph (a). Samples were equally divided over multiple gels which were derived and processed in parallel. Data is presented as box plots indicating median and interquartile range, with whiskers indicating min and max. p-value and significance is depicted between groups within each time-point *p < 0.05