Skip to main content

Advertisement

Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: CD248 and integrin alpha-8 are candidate markers for differentiating lung fibroblast subtypes

Fig. 4

Localization of Sca-1high fibroblasts and Sca-1low fibroblasts in adult mouse lungs. Hoechst 33342 (a), lineage-specific cell surface markers (CD31, CD45, CD146, E-cadherin, LYVE1, and TER-119) (b), Sca-1 (c), and PDGFRA (d) staining, merged images (e). Mouse lungs were also stained with hematoxylin and eosin (H&E) (f) and Elastica van Gieson stain (EVG) (g); collagen fibers are indicated as red bundles, and elastic fibers are indicated as black bundles. Arrows in (a) to (e) indicate Sca-1highlinnegPDGFRAhigh fibroblasts localized in the adventitia of pulmonary artery. Hoechst 33342 (h), lineage-specific cell surface markers (i), Sca-1 (j), and ITGA8 (k) staining, merged images (l). Mouse lungs were also stained with H&E (m) and EVG (n). Arrows in (h) to (l) indicate Sca-1highlinnegITGA8low fibroblasts localized in the bronchovascular interstitium. Hoechst 33342 (o), lineage-specific cell surface markers and Sca-1 (p), ITGA8 (q), and PDGFRA (r) staining, merged images (s). Mouse lungs were also stained with H&E (t) and EVG (u). Arrows in (o) to (s) indicate Sca-1lowlinnegITGA8high fibroblasts localized in the adventitia of pulmonary vein. Arrows in (u) indicate elastic fibers in the adventitia of pulmonary vein. Br, bronchial epithelium. PA, pulmonary artery. PV, pulmonary vein. Representative results are presented in all panels; scale bars, 50 μm

Back to article page