Fig. 6

Serial sections of normal human lungs were stained with (a and f) hematoxylin and eosin (H&E) and (b and g) Elastica van Gieson stain (EVG). IHC of normal human lungs using (c and h) anti-CD248 antibody and (d, e, i, j) anti-ITGA8 antibody; a–e, small veins in alveolar region and f–j, alveolar septum. Multiplex immunofluorescent images of the small veins and the alveolar septum: (k) blue, DAPI; (l) green, lineage-specific markers; (m) yellow, CD248; (n) red, ITGA8; and (o) merged. White arrows in (k–o) indicate the nuclei of lin^negCD248^lowITGA8^high fibroblast-like cells located in the adventitia of the small vein and in the alveolar septum. Inset in panel (o) is an enlarged figure of the dotted rectangle of the alveolar septum; the white arrows indicate the nuclei of lin^negCD248^lowITGA8^high fibroblast-like cell shown in the inset. V, small vein. Representative results are presented in all panels; scale bars, 50 μm (a–d, f–i), 20 μm (e and j), 100 μm (k–o)