Fig. 5

GAB1 is a target gene of miR-34b-5p. (a) GAB1 was predicted as a downstream target of miR-34b-5p using TargetScan. The putative GAB1 binding site for miR-34b-5p (GAB1-WT) and the designed mutant sequence (GAB1-MUT) were shown. (b) HEK-293 T cells co-transfected with miR mimics (or miR-34b-5p mimics) and GAB1-WT (or GAB1-MUT) were analyzed for luciferase activity 48 h after transfection using dual-luciferase reporter assay. (c) PMVECs were transfected with miR mimics or miR-34b-5p mimics. The expression of GAB1 in transfected PMVECs were examined using Western blot. (d) The relative expression of GAB1 in sham- and CLP-operated animals were assessed using qRT-PCR. (E) The correlation between miR-34b-5p level and GAB1 expression in mouse lung tissues were examined using Spearman’s rank correlation test. (F) The protein expression of GAB1 in mice was measured using Western blot