Skip to main content
Fig. 6 | BMC Pulmonary Medicine

Fig. 6

From: LncRNA TUG1 alleviates sepsis-induced acute lung injury by targeting miR-34b-5p/GAB1

Fig. 6

TUG1 regulated sepsis-induced apoptosis and inflammation via the modulation of miR-34b-5p and GAB1. PMVECs were divided into 3 groups: Vector+miR mimics, TUG1 + miR mimics and TUG1 + miR-34b-5p mimics. Cells in Vector+miR mimics group were co-transfected with control adenoviral vector and control mimics. PMVECs in TUG1 + miR mimics and TUG1 + miR-34b-5p mimics groups were co-transfected with adenoviral vector expressing TUG1 and control mimics (or miR-34b-5p mimics). LPS stimulation was performed 24 h post transfection. (a) The protein levels of GAB1, Bax, Bcl-2, and cleaved caspas-3, and cleaved PARP in all groups were determined using Western blot. Representative plots (left) and bar charts (right) were shown. (b) The production of TNF-α, IL-1β and IL-6 in co-transfected PMVECs were measured using ELISA

Back to article page